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In 1871 the prominent Russian doctor V.A. Manassein published his report in which he described his numerous experiments with “penicillium glaucum”. During his observations of this particular plant V.A. Manassein discovered that many bacteria did not multiply and did not produce new ones in its presence. Due to this phenomenon V.A. Manassein began to investigate the properties of this plant. He determined that it grew well only in such an environment which was provided with oxygen, that is it was aerobic. The young culture of penicillium glaucum destroyed bacteria rapidly, they could not multiply and even disappeared in its presence. The great significance of penicillium glaucum as a protective agent against bacterial infection was clearly determined.
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The young culture of penicillium glaucum destroyed bacteria rapidly, they could not multiply and _______.
was clearly determined
was provided with oxygen, that is it was aerobic
did not produce new ones in its presence
even disappeared in its presence